> Columnas HPLC | Exclusión por Tamaño
< Volver a: Aqueous SEC
KW400
High Performance Semi-micro Aqueous SEC (GFC)
KW400
> Reducing particle size of the packing material enhances column performance
> Three- or four-fold higher sensitivity than KW-800 series
> KW405-4F is applicable to samples with a molecular weight above 1,000,000
> Corresponds to USP L20, L33, and L59
(High Performance Semi-micro columns)
Product code
F6989201
F6989202
F6989203
F6989204
F6700132
Product name
KW402.5-4F
KW403-4F
KW404-4F
KW405-4F
KW400G-4A
Plate number
(TPN/column)
≥ 35,000
≥ 35,000
≥ 25,000
≥ 25,000
Guard column
Particle size
(μm)
3
3
5
5
5
Size (mm)
I.D. x Length
4.6 x 300
4.6 x 300
4.6 x 300
4.6 x 300
4.6 x 10
Shipping
solvent
H2O
H2O
H2O
H2O
H2O
Product name
Max. usable
pressure
(MPa)
Recommended
flow rate
(mL/min)
Max. usable
flow rate
(mL/min)
KW402.5-4F
KW403-4F
KW404-4F
KW405-4F
KW400G-4A
10
10
7
7
-
0.35
0.35
0.35
0.35
-
0.5
0.5
0.5
0.5
-
*( ): Estimated value
Usable organic solvent concentration: Up to 100% for methanol and acetonitrile.
Use of the KW400 series with semi-micro type devices is recommended.
Usable temp. range (°C)
5 to 45
5 to 45
5 to 45
5 to 45
5 to 45
Usable
pH range
3.0 to 7.5
3.0 to 7.5
3.0 to 7.5
3.0 to 7.5
3.0 to 7.5
Usable
salt conc
≤ 0.3M
≤ 0.3M
≤ 0.3M
≤ 0.3M
≤ 0.3M
<Semi-micro columns> * The following semi-micro columns are made to order.
Product code
Product name
Particle size
(μm)
Size (mm)
I.D. x Length
F7781213
F7781212
KW402.5-4B
KW402.5-4D
3
3
4.6 x 50
4.6 x 150
F7781313
F7781312
KW403-4B
KW403-4D
3
3
4.6 x 50
4.6 x 150
[Target molecular weight range and Exclusion limit]
* Please use the above table as a rough indication for the column selection.
With Protein (eluent : phosphate buffer)
Product name
Target molecular weight range
KW402.5
KW403
KW404
KW405
5,000 - 70,000
10,000 - 500,000
30,000 - *(4,000,000)
200,000 - *(20,000,000)
*( ) Estimated value
Exclusion limit
150,000
600,000
*(4,000,000)
*(20,000,000)
With Pullulan (eluent : ultrapure water)
Product name
KW402.5
KW403
KW404
KW405
Target molecular weight range
2,000 - 40,000
3,000 - 50,000
20,000 - 300,000
100,000 - 700,000
Exclusion limit
60,000
80,000
400,000
1,300,000
Celular | WhatsApp: 318 213 4434
Calle 35A sur No. 26B 67 | Barrio Bravo Páez, Bogotá D.C. | Colombia
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